Photoactivated Dimerizers


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Strategies for induction of protein dimerization
Strategies for induction of protein dimerization. (a) Small-molecule or light-induced dimerizers conditionally interact with light. At top, two noninteracting proteins are forced together by a tethered compound that bridges the proteins. At bottom, a photoreceptor protein and a second protein are unable to interact in the dark, but light alters the conformation of the photoreceptor and enables interaction. (b) Schemes for regulation of target proteins with light. Target proteins or domains of interest (circles) can be fused to the dimerizer modules, allowing inducible dimerization of target proteins. In the top figure, the target proteins are inactive fragments of a split protein, which when dimerized result in reconstitution of functional protein. Alternatively, inducible dimerization can be used to force proteins to particular subcellular locations, where they may be active or sequestered (bottom figure). From [1]
Light-regulated induction of transcription
Light-regulated induction of transcription. In the transcriptional activation system described by Shimizu-Sato et al., interaction between PhyB and PIF3 was used to reconstitute a split Gal4 transcription factor. In this case, PhyB was fused to a Gal4-binding domain (GalBD) and PIF3 fused to a Gal4 activation domain (Gal4AD). In dark or far-red light, PhyB does not interact with PIF3 and transcription remains off. With red light, PhyB interacts with PIF3, reconstituting a functional Gal4 transcription factor and allowing transcriptional induction. From [1]


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  1. Error fetching PMID 22341323: [Tucker2012]


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