Electroporation

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[THIS PAGE NEEDS SOME FLESHING-UP AND REWORK]

In utero ventricular microinjection and electroporation

[Describe principle, how it compares to other techniques in terms of expression levels, what brain areas are amenable to in utero electroporation, protocol]

This technique was used successfully to express ChR2 in the following papers:

Links

Postnatal non-ventricular microinjection and electroporation

Links

Single-cell electroporation

The Häusser lab has developed a protocol for electroporating single of groups of neurons in vivo. This methods allows labeling very few neurons at precise locations within the brain. Satisfactory expression levels (in particular for ChR2 expression) appear to be reached only after 48 hours.

References

Error fetching PMID 21884798:
  1. Error fetching PMID 21884798: [Ako2011]