DLP Projection

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DLP projection (DLP: digital light processing), or in more simple words videoprojection, is based on an interesting set of techniques which can be used for light patterning in optogenetics. Briefly, the general idea is that commercially available videoprojectors can be relatively easily adapted to your optogenetic setup if you are a DIY (do it yourself) aficionado. Amongst existing projection technologies, LCD (liquid crystal display) and DLP projectors[1] have been tested so far for optogenetic stimulation and/or inhibition of neuronal activity in isolated preparations (acute brain slices or nematodes in a petri dish). These techniques have been described in methods papers (LCD projector: [2], DLP projector: [3]).

Modifying an LCD projector for multiwavelength patterned illumination

This strategy has been described in [2].

Optical configuration of the system and components. a: Final optical configuration for the system. The epifluorescence optics are replaced by an accessory tube lens (infinity corrected) or relay lens pair (160 mm) and a modified 3-LCD projector. b: Modifications of the 3-LCD projector to limit the spectral width of the RGB colors. Internal filters are added to the 3-LCD projector, thus narrowing the band pass for each RGB color. From [2].

Modifying a DMD-based projector for multiwavelength patterned illumination

This strategy has been described in [3].

The image made by a DMD-based projector is focused on the specimen through a focusing lens (L2), a half/dichroic mirror (HM/DM) and an objective lens. L1, projector lens. CP, conjugate plane. Here, d1 and d2 were, respectively, 70 and 90mm. From [3].


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  1. Here DLP is used to designate DMD-based projectors. DMD stands for digital micromirror device. A DLP projector can be equiped with one to three DMDs.


  2. Error fetching PMID 22240583: [Stirman2012]
  3. Error fetching PMID 22469653: [Sakai2012]
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